Dynamic cyclin profiles as a tool to segregate the cell cycle

Background and novelty Mammalian cells growth, productivity and cell death are highly regulated and coordinated processes. The cell cycle is at the centre of cellular control and should play a key role in determining optimization strategies towards improving productivity [1]. Specifically, cell productivity is cell cycle, cell-line and promoter dependant [2]. The cyclins are key regulators that activate their partner cyclin-dependent kinases (CDKs) and target specific proteins driving the cell cycle. To our knowledge, there is no information on cyclin phase-dependent expression profiles of industrial relevant mammalian cell lines. We use the cyclin profiles as a tool to identify and quantify the landmarks of the cell cycle and implement a modelling approach to describe the bioprocess. Hereby, we introduce two possible experimental approaches to obtain such dynamic cyclin profiles.